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Title:
CSPs: Substrate Induced Respiration from 12 CSPs, through the soil profile
Access rights:
Free for public
Usage rights:
Permission is granted to anybody to access, use and publish all open for public data freely. The commercial use of any data is prohibited. The quality and completeness of data cannot be guaranteed. Users employ these data at their own risk. In order to make attribution of use for owners of the data possible, the identifier of ownership of data must be retained with every data record. Users must publicly acknowledge, in conjunction with the use of the data, the data owners. Cite the data as follows: Wu, Y.T., Buscot, F., Gutknecht J. and Wubet, T. (2008): Substrate Induced Respiration from 12 CSPs, through the soil profile. BEF-China data portal (Accessed through URL http://china.befdata.biow.uni-leipzig.de/datasets/209)
Published:
Yu Ting Wu, Jessica Gutknecht, Karin Nadrowski, Christian Geißler, Peter Kühn, Thomas Scholten, Sabine Both, Alexandra Erfmeier, Martin Böhnke, Helge Bruelheide, Tesfaye Wubet, François Buscot; Relationships between soil microorganisms, plant communities and soil characteristics in Chines subtropical forests. Ecosystems (accepted Februrary 2012). doi:10.1007/s10021-012-9533-3
Abstract:
This is the 2008 CSP SIR data from a subset of 12 CSPs representing the age gradient. Samples were analyzed over the depth profile of each CSP (see detailed methods below) and analyzed for basal respiration, substrate induced respiration, and the quotient CO2, which represent the heterotrophic microbial biomass and ecophysiological capacity of the microbial community. The point of this study was to characterize the microbial community of the CSPs, as well as to determine which factors most influence microbial community dynamics, including forest age and other factors.
Design:
A subset of 12 CSPs were selected according to an successional age and a diversity gradient; based on the original age classifications which were later changed: young (CSPs 16,17,25,26), medium (CSPs 1,5,8,9) and old (CSPs 2,4,12,13). At each CSP, samples were originally taken by SP6 at 5 cm intervals down to 50 cm, and then combined according to soil horizons. For each depth, samples were taken from all 9 subplots then combined into one pooled sample.
Spatial extent:
The Gutianshan National Nature Reserve (NNR) is located in the western part of Zhejiang Province (29º8'18" – 29º17'29" N, 118º2'14" – 118º11'12" E, Fig. 1). The Gutianshan NNR has an area of approximately 81 km2 and was initially established as a National Forest Reserve in 1975 and became a National Nature Reserve in 2001. The NNR comprises a large portion of broad-leaved forests of advanced successional stages (Hu & Yu 2008), which have not been managed since the beginning of the 1990ies, as well as young successional stages and conifer plantations, mainly of Cunninghamia lanceolata and Pinus massoniana. --- The vegetation is composed of different types of subtropical evergreen and mixed broad-leaved forests (Yu et al. 2001). Most of the stands are secondary forests, evidenced by maximum tree ages of 180 years, by agricultural terraces in almost all plots and by the presence of charcoal in almost all soil profiles. Around the Gutianshan NRR extensive deforestation has occurred during the Great Leap Forward in the 1950s, as in most parts of Southeast China. However, due to prevailing steep slopes, the Gutianshan area was only marginally usable for agricultural activities, and thus an exceptionally intact forest cover has been preserved. --- The climate at Gutianshan NNR is warm and temperate with a short dry season in November and December and with warm summers (Fig. 2). The climatic conditions are characteristic for the subtropics with an annual average temperature of 15.1°C, January minimum temperatures of -6.8°C, July maximum temperatures of 38.1°C and an accumulated temperature sum (≥ 5°C) of 5221.5 degree days.
Temporal extent:
Samples were taken in August and September 2008 from the CSPs, taken to Germany, and analyzed in the laboratory afterward (over the next several months). Cmic was estimated with 25 g oven-dry equivalent of field-moist soil by substrate induced respiration. Before analyzing, frozen samples were completely thawed and adjusted to an appropriate moisture content. All samples were pre-incubated at 20 ˚C for at least 48 h. Later the analysis was also carried out at 20 ˚C. Basal respiration (BR) was first measured for 24 hours without amendment, followed by addition of glucose and talcum to measure substrate induced respiration (Cmic). The CO2 production of each sample was measured with an automatic infrared gas analyzer (Type 225 MK3) in an air flushed incubation device. The CO2 production was then averaged over four internal replicates of each sample. This CO2 production can be used to estimate the microbial biomass and to calculate two microbial ecophysiological indicators, the metabolic quotient (qCO2) and the Cmic/ Corg ratio. The qCO2 was determined as the ratio of BR (μg g soil-1 hr-1 CO2-C) to Cmic (μg g soil-1). The Cmic/ Corg ratio was calculated by dividing Cmic (μg) by percent soil organic carbon (Corg).
Taxonomic extent:
No specific organisms were analyzed here, but this data can represent the total microbial heterotrophic biomass
Measurement cirumstances:
No information available
Data analysis:
The parameters of interest were the basal respiration (BR, soil CO2 production with no ammendments), substrate induced respiration (SIR, soil CO2 production after addition of glucose and talc), and the quotient CO2 (qCO2, the ratio of BR/SIR). Data were then analyzed in R using multivariate (NMDS) or univariate (co-variate regressions and ANOVA) methods. The columns of CSP name, depth, and horizon name are explanitory variables, the response columns are the BR, SIR, and qCO2 --- note that the age classes analyzed here are not the most current age classes. They were changed, but analysis was already deep underway on the paper at that point.

Filter:
Dataset column

Name:
CSP
Definition:
BEF research plot name; Reasearch plots of the Biodiversity - Ecosystem functioning experiment (BEF-China). Comparative Study Plots (CSP) in the Gutianshan Nature Reserve, having a size of 30x30m^2, measured on the ground.
Unit:
No information available
Datagroup:
BEF research plot name
Keywords:
CSP
Values:
CSP02
CSP04
CSP01
CSP05
CSP08
Contributors:

Dataset column

Name:
lab_num
Definition:
laboratory number of laboratory of soil science and geoecology in Tübingen
Unit:
No information available
Datagroup:
Laboratory number of Laboratory of Soil Science and Geoecology in Tübingen
Keywords:
soil base
Values:
3177
3183
3178
3180
3179
Contributors:

Dataset column

Name:
sample_num
Definition:
field name of the sampleHelper
Unit:
No information available
Datagroup:
Helper
Keywords:
soil base
Values:
CSP01-P-1-3
CSP01-P-1-2
CSP01-P-1-4
CSP02-P-1-1
CSP01-P-1-1
Contributors:
No information available

Dataset column

Name:
depth
Definition:
depth increment of the soil sampledHelper
Unit:
No information available
Datagroup:
Soil depth
Keywords:
soil depth
Values:
10-30cm
0-10cm
10-20cm
0-5cm
20-30cm
Contributors:
No information available

Dataset column

Name:
horizon
Definition:
the soil horizon name assigned by SP6 to that depth incrementHelper
Unit:
No information available
Datagroup:
Soil horizon
Keywords:
soil horizon
Values:
Ah-Bv
Ah
Bv
Ah+Al-Bv
Ah+Bv
Contributors:
No information available

Dataset column

Name:
age class
Definition:
the age class assigned at the time of samplingHelper
Unit:
No information available
Datagroup:
Successional age of a forest plot
Keywords:
No information available
Values:
Old
Young
Medium
Contributors:
No information available

Dataset column

Name:
BR
Definition:
Basal respiration; CO2 production of the soil samples after 24 hours, with no ammendment
Unit:
µg g soil-1 hr-1 CO2-C
Datagroup:
Substrate Induced respiration
Keywords:
soil, SIR, respiration
Values:
0.26
0.02
0.24
0.3475
0.285
Contributors:
No information available

Dataset column

Name:
SIR
Definition:
Substrate Induced respiration (SIR, or Cmic). SIR is defined as the CO2 production with glucose ammendment.
Unit:
µg Cmic g soil-1
Datagroup:
Substrate Induced respiration
Keywords:
soil, biomass, SIR
Values:
101.58
101.98
101.9
101.39
109.75
Contributors:
No information available

Dataset column

Name:
qCO2
Definition:
quotient CO2; defined as BR divided by SIR
Unit:
ng C µg Cmic-1 hr-1
Datagroup:
Substrate Induced respiration
Keywords:
soil, SIR, respiration
Values:
13.02747791952895
10.344912117275024
12.079599674153382
1.047943411055803
13.218098627351296
Contributors:
No information available

No information available


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Attachment:

File name:
CSP4all_new2.xls
Description:
Updated file including functional diversity measures
Uri:
No information available
Is essential:
No information available
Content type:
application/vnd.ms-excel
File size:
108 KB
Created at:
2012-03-22
Updated at:
2012-03-22

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32 wubet medium

Tesfaye
Wubet

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