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Title:
Main Experiment: Ammonium concentrations and ammonification rates in soils (Site A, Ext. A, B and E plots)
Access rights:
Free within projects
Usage rights:
Usage rights for SP5e members
Published:
No information available
Abstract:
Soil sampling was carried out on a subset of 100 plots of two overlapping extinction scenarios on Site A (Extinction scenario A and B). The sampüles were collected from young forest communities composed of 1 - 24 species (see general plot information concerning community compositions of the BEF-China experiment: http://china.befdata.biow.uni-leipzig.de/datasets/71; Species nomenclature following the Flora of China). The species composition covers two overlapping species pools and also includes two commercially relevant tree species monocultures (Extinction scenario E, Pinus massoniana and Cunninghamia lanceolata). Moreover, we also sampled plots without trees. Sampling was carried out in July 2015.
Design:
Within the plots, we collected soil samples from 12 random positions which followed a frame around the central 8 x 8 trees of every plot. Soil cores with a diameter of 13 mm were taken from the first ten centimeters of the top layer. These subsamples were pooled plotwise, freshly sieved in 2 mm fraction, homogenized and stored in the fridge for analyses.
Spatial extent:
Plots of Exctinction scenario A and B (http://china.befdata.biow.uni-leipzig.de/datasets/71). 100 plots were sampled, including non-tree community plots and monocultures of Pinus massoniana and Cunninghammi lanceolata (Extinction Scenario E).
Temporal extent:
Sampling was carried out throughout July 2015.
Taxonomic extent:
No tree species were included in the analyses. However, soil cores were taken from communities comprising the following species (and their mixtures according to (http://china.befdata.biow.uni-leipzig.de/datasets/71): Nyssa sinensis Castanea henryi Quercus fabri Lithocarpus glaber Quercus serrata Koelreuteria bipinnata Choerospondias axillaris Castanopsis sclerophylla Triadica sebifera Liquidambar formosana Sapindus saponaria Schima superba Castanopsis eyrei Cyclobalanopsis myrsinifolia Rhus chinensis Cyclobalanopsis glauca Castanopsis carlesii Melia azedarach Triadica cochinchinensis Diospyros japonica Acer davidii Cinnamomum camphora Daphniphyllum oldhamii Quercus acutissima Pinus massoniana Cunninghamia lanceolata
Measurement cirumstances:
No information available
Data analysis:
Nitrogen mineralization rates were determined from 10 g fresh soil extracted with 100 ml 1 M KCl. After reacting with a sodium salicylate solution to a indophenol colour complex (Kandeler and Gerber 1988; Niklaus et al. 2001) NH4+ concentrations of the filtered extracts were measured colorimetrically (Microplate Reader Synergy Mx, BioTek, Winooski, United States). Another subsample of 10 g fresh soil was incubated at 40 °C in glas vials for seven days. To ensure anaerobic condition, the soil was water-logged with 15 ml H2O and vials were sealed with a crimp-cap to reduce oxygen exchange to a minimal level (Niklaus et al. 2001). Under anaerobic conditions, soil microbes only produce NH4+ instead of NO3- (Niklaus et al. 2001). After incubation, we filtered and extracted the samples with 85 ml 1.176 M KCl to ensure similar molarity to the non-incubated samples. Ammonium analyses were done analogous to the non-incubated samples. N-mineralization rates were calculated as the difference of the ammonium concentration in the incubated and non-incubated sample divided by the number of days of incubation (Niklaus et al. 2001).

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Dataset column

Name:
plot
Definition:
plot samples were taken
Unit:
No information available
Datagroup:
Plot name Main experiment
Keywords:
No information available
Values:
E24
C32
BC36
E22
E23
Contributors:
No information available

Dataset column

Name:
site
Definition:
site samples were taken
Unit:
No information available
Datagroup:
Experimental Site
Keywords:
No information available
Values:
A
Contributors:
No information available

Dataset column

Name:
div
Definition:
plot tree species richness
Unit:
tree species
Datagroup:
Tree species richness
Keywords:
No information available
Values:
24
0
1
16
2
Contributors:
No information available

Dataset column

Name:
NH4fresh
Definition:
NH4fresh
Unit:
No information available
Datagroup:
Ammonium
Keywords:
No information available
Values:
0.5565020770503535
0
0.17751165123001583
0.7054992764109983
10.32407900748706
Contributors:
No information available

Dataset column

Name:
NH4inc
Definition:
NH4inc
Unit:
No information available
Datagroup:
Ammonium
Keywords:
No information available
Values:
46.06215846994536
44.08858526236796
43.998271146834675
45.71990490016505
42.70546772286854
Contributors:
No information available

Dataset column

Name:
NH4rate
Definition:
ammonium production in the incubated soil per day, concentrations given in µg ammonium per g soil (dry mass) per day
Unit:
µg/g soil * d
Datagroup:
Ammonification rate
Keywords:
No information available
Values:
10.017887311246996
11.040048919685697
10.190714317286321
10.01089322598524
10.18286433431473
Contributors:
No information available

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94 seidelmann medium

Katrin
Leppert (ehem. Seidelmann)

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Jürgen
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Pascal
Niklaus

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